Chemical Reprogramming Kit
BeiCell™ Human Chemical Reprogramming Induced Pluripotent Stem Cell Kit
Product Name: BeiCell™ Human Chemical Reprogramming Induced Pluripotent Stem Cell Kit
Catalog Number: BCTCiPS-001
Specification: 1 kit, containing 6 media.
Composition & Storage
Storage Conditions
The kit is shipped on dry ice. When placing an online order, dry ice shipping must be selected.
Store at –15 ℃ to –25 ℃. Avoid repeated freezing and thawing. After thawing, store at 2–8 ℃ for up to 3 weeks.
Product Use
Cellular reprogramming tools enable manipulation of cell identity, allowing generation of desired cell types with broad applications in disease modeling, drug discovery, and regenerative medicine. Chemical reprogramming synergistically targets cell signaling pathways and epigenetic modifiers without genetic manipulation, offering significant advantages for pharmacological and therapeutic development. Recently, a scalable, efficient, and rapid chemical reprogramming method was reported for inducing human somatic cells into pluripotent stem cells (Guan et al., Nature, 2022; Liuyang et al., Cell Stem Cell, 2023).
This kit, optimized from a patent application (METHODS FOR CHEMICAL REPROGRAMMING AND PLURIPOTENT STEM CELLS, PCT/CN2022/124960) and related publication (Liuyang et al., Cell Stem Cell, 2023), enables generation of human chemically induced pluripotent stem cells (hCiPSCs) from human adult adipose-derived stromal cells (hADSCs) or human dermal fibroblasts (HDFs).
Advantages
Simple: A user-friendly, easy-to-operate induction process which only involves 4 key steps by simply changing the medium as per the user's manual.
Efficient: Cell reprogramming efficiency ranges from 0.4% to 6.8% for skin fibroblasts and 1% to 31% for adipose derived stromal cells.
Stable: Efficient induction of somatic cells from diverse genetic back- grounds and various age groups.
Easy Standardization: Defined composition, serum-free and feed- er-independent, easy to standardize
Safe: The utilization of chemical small molecules avoids genome integration, non-immunogenic, safer than transgenic techniques.
This product is designated for research purposes exclusively and is not intended for utilization in diagnostic or any other commercial applications. Multilateral Trade Agreements (MTA) dictate that you must refrain from engaging, either directly or indirectly, in the commercial utilization of these materials. This includes scenarios such as conducting research subject to consultancy or licensing obligations with third parties, screening compound libraries, or developing commercial products.
Reference
Guan, J., Wang, G., Wang, J., Zhang, Z., Fu, Y., Cheng, L., Meng, G., Lyu, Y., Zhu, J., Li, Y., et al. (2022). Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605, 325-331.
Liuyang, S., Wang, G., Wang, Y., He, H., Lyu, Y., Cheng, L., Yang, Z., Guan, J., Fu, Y., Zhu, J., et al. (2023). Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. Cell stem cell 30, 450-459.e459.
Product Name: BeiCell™ Human Chemical Reprogramming Kit (2nd Generation)
Catalog Number: BCTCiPS-002
This upgraded version is based on the latest research — A Rapid Chemical Reprogramming System to Generate Human Pluripotent Stem Cells — published in Nature Chemical Biology (January 3, 2025) through collaboration between the Deng Hongkui Lab (Peking University, Changping National Laboratory) and the Guan Jingyang Lab (Peking University). The study identified key epigenetic barriers and further optimized the rapid chemical reprogramming system, reducing the time required for efficient hCiPSC generation from 30 days to under 16 days, with a minimum of just 10 days.
Validated on somatic cells from 15 donors of diverse genetic backgrounds and ages, this method consistently achieved high induction efficiency, with peak efficiency reaching 38%. For donor cells previously resistant to reprogramming, this rapid system increased the 16-day reprogramming efficiency by more than 20-fold, significantly enhancing applicability across individuals.
Advantages
Simple Operation: Reprogramming is completed via straightforward media changes.
High Efficiency: Induction within 20 days, with peak efficiency up to 38%.
Broad Applicability: Effective for somatic cells from various genetic backgrounds and ages; highly stable.
Safe & Reliable: Small molecules avoid genomic integration and are non-immunogenic, safer than transgenic methods.
Standardized Production: Defined components, serum-free, feeder-free; facilitates standardized protocols.
hCiPSC Generation Workflow
Chemical reprogramming does not require complex techniques such as viral/plasmid transduction or electroporation. Simple media changes, morphological observation, and passaging efficiently drive the conversion of human somatic cells into hCiPSCs.
Key Steps:
Starter Cell Seeding (Starter Cell Seeding Medium)
Phase I Induction (Medium A)
Phase II Induction (Medium B)
Phase III Induction (Media C, D, and E)
Single-Cell Passage Culture (Medium F)
hCiPSC Passage Culture (hCiPSC Culture Medium)
Composition
References
Wang, Y. et al. A rapid chemical reprogramming system to generate human pluripotent stem cells. Nat Chem Biol 1–9 (2025) doi:10.1038/s41589-024-01799-8.
Liuyang et al., Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. Cell Stem Cell 30, 450-459.e9 (2023).
Guan et al., Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605, 325–331 (2022).
Product Name: BeiCell™ Human Blood Cell Chemical Reprogramming Kit
Catalog Number: BCTCiPS-003
Specification: 1 kit, containing 8 media.
Description
The BeiCell™ Human Blood Cell Chemical Reprogramming Kit reprograms human peripheral blood mononuclear cells into chemically induced pluripotent stem cells.
By optimizing formulations and incorporating stage-specific small molecule combinations into dedicated media, this kit is widely applicable for stem cell and regenerative medicine research, disease modeling, and drug screening.
Advantages
Genetic Safety: No risk of proto-oncogene integration.
Simple Operation: User-friendly culture protocol.
Reliable Results: Consistent and reproducible across batches.
Convenient Source Material: Requires only peripheral blood.
Reference
Peng, F. et al. Chemical reprogramming of human blood cells to pluripotent stem cells. Cell Stem Cell 32, 1192-1199 e1111 (2025). https://doi.org/10.1016/j.stem.2025.07.003
Contact Us
Ms. Fan, Tel: 13426082940, Email: selina@cy-tech.com.cn
