Chemical Reprogramming Kit
Introduction
Cellular reprogramming tools can manipulate cell identity, enabling the generation of desired cell types that provide broad applications in disease modeling, drug discovery and regenerative medicine. Chemical reprogramming approach synergistically targets cell signaling pathways and epigenetic modifiers without gene manipulation, and offers considerable advantages for the development of pharmacological and therapeutic strategies. Recently, a new scalable, efficient, and rapid chemical reprogramming approach was reported, which can induce human somatic cells into pluripotent stem cells (Guan, J et al., Nature, 2022; Liuyang, S et al., Cell Stem Cell, 2023). This kit, optimized from a patent in filing (METHODS FOR CHEMICAL REPROGRAMMING AND PLURIPOTENT STEM CELLS, PCT/CN2022/124960) and publication (Liuyang, S et al., Cell Stem Cell, 2023), can be used to generate human chemically induced pluripotent stem cell (hCiPSCs) from human adult adipose-derived stromal cells (hADSC) or skin dermal fibroblasts (HDFs).
Product Specifications
Simple: A user-friendly, easy-to-operate induction process which only involves 4 key steps by simply changing the medium as per the user's manual.
Efficient: Cell reprogramming efficiency ranges from 0.4% to 6.8% for skin fibroblasts and 1% to 31% for adipose derived stromal cells.
Stable: Efficient induction of somatic cells from diverse genetic back- grounds and various age groups.
Easy Standardization: Defined composition, serum-free and feed- er-independent, easy to standardize
Safe: The utilization of chemical small molecules avoids genome integration, non-immunogenic, safer than transgenic techniques.
Table: Kit contents (Cat# HST-crkit-001) (BCTCiPS-001)
Bottle | Label | Content | Function/ description |
1 | Medium A | 1 bottle, 100 mL | Medium for Stage 1 induction |
2 | Medium B | 1 bottle, 100 mL | Medium for Stage 2 induction |
3 | Medium C | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 0~4) induction |
4 | Medium D | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 4~8) induction |
5 | Medium E | 1 bottle, 30 mL | Medium for Stage 3 induction (Day 8~12) induction |
6 | Medium F | 1 bottle, 100 mL | Medium for hCiPS cell line derivation |
Storage Conditions
The kit is shipped on dry ice. When placing an online order, it is mandatory to select Dry Ice shipping.
When stored at -15 to -25°C. Avoid repeated freezing and thawing. After thawing, store for up to 3 weeks at 2 to 8 °C .
Product Use
This product is designated for research purposes exclusively and is not intended for utilization in diagnostic or any other commercial applications. Multilateral Trade Agreements (MTA) dictate that you must refrain from engaging, either directly or indirectly, in the commercial utilization of these materials. This includes scenarios such as conducting research subject to consultancy or licensing obligations with third parties, screening compound libraries, or developing commercial products.
Contact Us
Ms. Fan, Tel: 13426082940, Email: selina@cy-tech.com.cn
Reference
Guan, J., Wang, G., Wang, J., Zhang, Z., Fu, Y., Cheng, L., Meng, G., Lyu, Y., Zhu, J., Li, Y., et al. (2022). Chemical reprogramming of human somatic cells to pluripotent stem cells. Nature 605, 325-331.
Liuyang, S., Wang, G., Wang, Y., He, H., Lyu, Y., Cheng, L., Yang, Z., Guan, J., Fu, Y., Zhu, J., et al. (2023). Highly efficient and rapid generation of human pluripotent stem cells by chemical reprogramming. Cell stem cell 30, 450-459.e459.
